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Image Search Results
Journal: The Journal of Physiology
Article Title: Activation of TRPC channels contributes to OA-NO 2 -induced responses in guinea-pig dorsal root ganglion neurons
doi: 10.1113/jphysiol.2014.271783
Figure Lengend Snippet: A, OAG (50 μm), a TRPC3/6/7 agonist, and O-N (30 μm) applied for 30 s to the same coverslip evoked transients in the same neurons; however, the OAG responses were smaller in amplitude than the O-N responses. B, La3+ (50 μm) reversibly blocked the OAG response. C and D, SKF-96365 (25 μm), a TRPC channel blocker, applied 3 min before and during the application of O-N reduced the amplitude of the O-N response (C) and the percentage of neurons responsive to O-N (D). E, several agonists – capsaicin (Cap,1 μm) applied for 15 s, AITC (100 μm) applied for 30 s, 4-αPDD (2 μm, a TRPV4 channel agonist) applied for 30 s, menthol (100 μm) applied for 30 s or OAG (50 μm) applied for 30 s – were applied to the same coverslip prior to the application of O-N. The number of neurons responding to each agonist is shown above each bar as a fraction of O-N responsive neurons. n represents the number of animals used for each set of experiments. OAG has the highest overlap with O-N.
Article Snippet: [ PMC free article ] [ PubMed ] Quick K, Zhao J,
Techniques:
Journal: International Journal of Molecular Sciences
Article Title: TRPC3 Is Downregulated in Primary Hyperparathyroidism
doi: 10.3390/ijms25084392
Figure Lengend Snippet: Immunohistochemical staining with an anti-TRPC3 antibody of the human parathyroid gland with and without image analysis. The upper two panels ( A , B ) illustrate microphotographs from healthy human parathyroid tissue. The lower two panels ( C , D ) present microphotographs from human parathyroid tissue from a patient with primary hyperparathyroidism (pHPT). Panels ( A , C ) are without image analysis and panels ( B , D ) are with image analysis. Panels ( B , D ) show semiquantitative diaminobenzidine tetrahydrochloride (DAB) color scoring. The color red represents a DAB score of 3, orange a DAB score of 2, and yellow a DAB score of 1. The color green represents subtracted connective and fatty tissues. The color blue shows the subtracted hematoxylin area. Both were attributed as DAB-negative. Panels ( A – D ) have 40× software magnification and a scale bar of 50 µm.
Article Snippet: However, according to the HPA and consensus datasets from the
Techniques: Immunohistochemical staining, Staining, Software
Journal: International Journal of Molecular Sciences
Article Title: TRPC3 Is Downregulated in Primary Hyperparathyroidism
doi: 10.3390/ijms25084392
Figure Lengend Snippet: Immunohistochemical staining with an anti-TRPC3 antibody of the human parathyroid gland. The left column ( A , C , E ) illustrates microphotographs from healthy human parathyroid tissue. The right column ( B , D , F ) presents microphotographs from human parathyroid tissue from patients with primary hyperparathyroidism (pHPT). Panel ( A ) displays an overview of healthy parathyroid tissue with a pool of chief cells on the right side and a pool of oxyphilic cells on the left side of the demarcation (scale bar: 100 µm). Panel ( C ) displays both chief (black arrow) and oxyphilic (white arrow) cells at higher magnification (scale bar: 50 µm). Panel ( B ) displays an overview of human parathyroid tissue from a patient with pHPT (scale bar: 100 µm). Panel ( D ) displays chief cells (black arrow) at higher magnification (scale bar: 50 µm). Panel ( E ) displays an overview of the negative control staining of healthy parathyroid tissue. Chief (black arrow), oxyphilic (white arrow), and fat cells (asterisk) are displayed (scale bar: 100 µm). Panel ( F ) displays an overview of the negative control staining of parathyroid tissue from a patient with pHPT (scale bar: 100 µm).
Article Snippet: However, according to the HPA and consensus datasets from the
Techniques: Immunohistochemical staining, Staining, Negative Control